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. 2009 Aug 19;4(8):e6667. doi: 10.1371/journal.pone.0006667

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Sander et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Conditions for the separation of the chromosomes and DIG labeling and detection of the var2csa DBL3X domain-derived probe sequence are given in the Materials and Methods. Gel measuring rulers and superimposed alignment lines are shown. Marker chromosomes are from Hansenula wingei. Note that the two uppermost faint bands are not chromosomes but a PFG artifact. HB3, 3D7, FCR3/It4 and 7G8 are long term laboratory isolates whilst Tz745, Tz748, Tz752, Tz755, Tz788 and Tz796 are recent field isolates from Korogwe, Tanzania.