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PfMSP3 sequence variation in the Zungarococha community. A, dHPLC elution chromatograms show that adapting dHPLC to screen for PfMSP3 variation can detect sequence differences of between 1 and 37 nucleotide differences, as evident by the secondary elution peak (arrows). B, Screening 630 distinct P. falciparum infections yielded only 10 mutations in PfMSP3. A total of 86 dHPLC-negative samples were sequenced to ensure adequate sensitivity.
