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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Clin Cancer Res. 2008 Dec 1;14(23):7804–7812. doi: 10.1158/1078-0432.CCR-08-0744

Figure 2. RFLP-PCR analysis of EAC samples.

Figure 2

Genomic DNA from primary EAC samples and a control samples was amplified by PCR, digested by SCRF1, and analyzed by gel electrophoresis. G/G homozygotes are represented by a single band at 146 bp, A/A homozygotes by a single band at 167 bp, and G/A heterozygotes by the presence of both bands. The 21 bp fragment can not be seen on this gel. Control samples were included in triplicate, along with the negative controls: control sample without enzyme and a no-template control. Genotypes were verified by TaqMan PCR.