Figure 5.

aPKCζ/λ/ι is activated in nephrin-deficient human podocytes. (A) Upregulation of phospho-aPKCζ/λ/ι(Thr410/403) is seen in nephrin-deficient podocytes on Western blot. Equal protein loading was verified by Ponceau S staining and reprobing with an antibody against total aPKCζ/λ/ι, which shows equivalent expression between WT and MT podocytes. (B) Immunofluorescence staining of cultured human podocytes shows nuclear sequestration of phospho-aPKCζ/λ/ι(Thr410/403) (white arrows, a) in WT podocytes and cytoplasmic expression in nephrin-deficient podocytes (MT; b). Re-introduction of nephrin in podocytes (MT+N) rescues the phenotype to predominantly nuclear expression (c). Magnification, ×40.