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. 2009 Aug;20(8):1733–1743. doi: 10.1681/ASN.2008111219

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Copyright © 2009 by the American Society of Nephrology

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Figure 8. — aPKCζ and 14-3-3θ bind cytosolic nephrin (AA 1057 through 1241). (A) Successful glutathione precipitation of native aPKCζ from MDCK cells by cytosolic nephrin (AA 1057 through 1241). Blots were probed with aPKCζ (rabbit polyclonal). Lanes 1 and 2, GST alone, no precipitation of 14-3-3θ (lane 2); lanes 3 and 4, precipitation of endogenous 14-3-3θ by the GST-cytosolic nephrin (AA 1057 through 1241) fusion protein in lane 4. (B) Successful glutathione precipitation of native 14-3-3θ from MDCK cells by cytosolic nephrin (AA 1057 through 1241). Blots were probed with 14-3-3θ (rabbit polyclonal; Santa Cruz Biotechnology). Lanes 1 and 2, GST alone, no precipitation of 14-3-3θ (lane 2); lanes 3 and 4, precipitation of endogenous 14-3-3θ by the GST-cytosolic NPHN (AA 1057 through 1241) fusion protein (lane 4).