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. 2009 Aug;20(8):1714–1723. doi: 10.1681/ASN.2008060640

Figure 2.

Figure 2.

Expression of CXCR4 in the developing kidney. CXCR4 was stained red (Alexa555) in the kidney sections of wild-type mice at E13.5 (A) and E17.5 (B through G). (A and B) Whole sections of the kidneys under low magnification revealed that CXCR4 is mainly expressed in the nephrogenic zone. (C) Co-immunostaining for cited1, a marker for CM, in green (Alexa488; left: CXCR4, middle: merged image) shows that CXCR4 is expressed in CM (arrows in inset) and PA (arrowheads in inset). Sections of CXCR4 null kidney are shown as negative control for CXCR4 staining (right). (D through F) Another section was co-immunostained for VEGFR2 in green (Alexa488; left: CXCR4, right: merged image). (D) CXCR4 was first detected only weakly in the endothelial cells in the cleft of the comma-shaped body (arrows) and more intensely in those at a basal position (arrowheads). (E) CXCR4 was detected in arterioles leading to the primitive glomeruli (arrows). Weak expression was detected in a stalk of glomerular endothelial cells (arrowheads). (F) In more mature glomeruli, CXCR4 was expressed in many glomerular endothelial cells. (G) Co-immunostaining for renin in green (Alexa488) shows that renin is expressed in pericytes surrounding CXCR4-positive endothelial cells, suggesting that CXCR4-positive vessels are afferent arterioles. Glm, glomerulus. Magnifications: ×100 in A and B; ×400 in C and E; ×600 in D, F, and G.