Figure 9.

IL-10⁺ Tregs inhibit kidney IRI in lymphocyte-deficient RAG-1 KO mice. RAG-1 KO mice were administered PBS or 1 × 10⁶ WT CD4⁺CD25⁺ Tregs, 1 × 10⁶ IL-10 KO CD4⁺CD25⁺ Tregs, or 1 × 10⁶ WT CD4⁺CD25⁻ cells 18 h before 28 min of bilateral renal ischemia or sham surgery. (A) After 24 h of reperfusion, renal function was estimated by measurement of PCr, and ATN was assessed by H&E staining. (B–E) Data are means ± SEM; values with different superscripts are significantly different from each other. P < 0.05; n = 4 to 9 per group (A). Magnifications: ×200 in B through E; ×600 in insets.