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. 2009 May 29;389(1-3):17–33. doi: 10.1016/j.jmb.2009.03.051

Fig. 4.

Fig. 4

Calorimetric analysis of the interaction of E. coli PNPase and RNase E recognition micro-domain (residues 1021–1061). Top panel: The isothermal calorimetry profile showing the heat released upon titrating RNase E micro-domain with PNPase core. Bottom panel: The integrated heats after correction for heat of dilution. The data are best fit with a single binding site model, yielding parameters N =  1.02  ±  0.02, Ka 9.56 ( ±  0.71) × 105 M-1, ΔH =  –21.48  ±  0.52 kcal mol–1, ΔS = -44.7 cal mol–1 K–1.