Figure 4.

Th1/Th17 phenotype of responding flu-specific IL-10 KO T cells. 2×10⁶ naïve WT or IL-10 KO HNT cells were transferred to WT or IL-10 KO hosts, respectively, then infected with 1LD₅₀ A/PR8. 7 d post-challenge, lung HNT cells were analyzed for (A) IFNγ or (B) IL-17 +/− peptide stimulation (n=5). (C) Peptide-dependent IL-17 production from WT or IL-10 KO HNT cells transferred to WT or IL-10 KO hosts (n=3, and * = P < 0.05). WT or IL-10 KO mice were challenged with 1LD₅₀ A/PR8 and ELISPOT analysis performed on d 7 for IL-17. (D) CD4 and CD8 peptide-specific, and (E) non-antigen elicited IL-17 spots per lung (n=5, and * = P < 0.05). (F) WT mice were treated with CD4 depleting or isotype control antibodies and infected with 1 LD₅₀ A/PR8. On d 7, lungs were analyzed for stated cytokines (n=5, * = P < 0.05 and ** = P < 0.01). A-C are representative of 3 independent exp, E-F of 2.