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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7353–7363. doi: 10.4049/jimmunol.0900657

Figure 6.

Figure 6

Th17-polarized effectors protect against lethal flu challenge employing novel mechanisms. 5×106 Th1 or Th17 HNT effectors were transferred to naïve BALB/c mice subsequently infected with 2 LD50 A/PR8 (10,000 EID50) and (A) conditional survival monitored (n=10 mice per group). (B) On stated days, viral titers were determined by quantitative PCR (n=5 mice / group / day, ** = P < 0.01, and *** = P < 0.001). (C) Respiratory rate and minute volume was determined as described (n=10 mice / group / time-point, * = P < 0.05, ** = P < 0.01, and *** = P < 0.001). (D) 5×106 Th17 effectors generated from WT or INFγ-/Perforin KO HNT cells were transferred to nude or JHD hosts, respectively. All mice were subsequently infected with a lethal dose (1500 EID50) of A/PR8. Conditional survival is shown (n=10 mice / group). A-C representative of at least 2 independent exp, D is a summary of 2 independent exp for each condition, with n=5 mice per group.