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. 2009 Jun 30;161(2-3):572–577. doi: 10.1016/j.neuroscience.2009.03.047

Fig. 1.

Fig. 1

RT-PCR analysis of NAPE-PLD gene expression. (A) Agarose gel electrophoresis of RT-PCR products for GAPDH (370 bp) from cDNA made to RNA from brain, heart, DRG and cultures prepared from DRG (lanes 1, 2, 3, and 4, respectively). (B) Agarose gel electrophoresis of RT-PCR products for NAPE-PLD (222 bp) from cDNA made to RNA from brain, heart, DRG and cultures prepared from DRG (lanes 1, 2, 3, and 4, respectively). Note that NAPE-PLD is expressed in all the tissues we examined. (C) NAPE-PLD (lane 1) and GAPDH (lane 4) gene expression in mouse dorsal root ganglion cultures grown under control conditions (without capsaicin). Lane 2 shows NAPE-PLD expression in a brain sample collected from the same animal used to derive the cultures analyzed in lanes 1 and 4. Lane 3 is a control PCR reaction, where RNA equivalent to the cDNA used in lane 1 has been used as template. (D) NAPE-PLD (lane 1) and GAPDH (lane 4) gene expression in mouse dorsal root ganglion cultures grown in the presence of 10 μM capsaicin overnight. Lane 2 shows NAPE-PLD expression in the brain sample collected from the same animal. Lane 3 is a control PCR reaction, where RNA equivalent to the cDNA used in lane 1 has been used as template. Note that the treatment of cultures with capsaicin significantly downregulated NAPE-PLD expression (lane 1 in C and D.)