Fig. 7.

LOX-1 deficiency does not impair the ability of oxLDL to inhibit macrophage apoptosis. (A) BMDM from wild-type mice (open bars) or LOX-1 −/− mice (solid bars) were seeded at 3 × 10³ cells/well in 96 well plates and cultured for 24 h. They were then washed and incubated for 24 h without M-CSF but with the indicated concentrations of ox-LDL. Viability was then measured by the bioreduction of MTS (described in Materials and Methods) and expressed as a percentage of that in control cells treated with M-CSF. (B) BMDM were plated at 10⁶ cells/well in six well plates and incubated for 24 h in medium containing indicated concentration of oxLDL but no M-CSF. The sub-diploid population was measured by flow cytometry after propidium iodide staining. Results are expressed relative to control cells incubated without CSF, which typically amounted to about 40% of cells. In both panels, results are the means±SD of pooled data from three independent experiments. None of the differences between wild-type and LOX-1 −/−.macrophages was significant.