Fig. 1.
ADRP overexpression decreases the loss of fatty acids from TAG and inhibits PPARα activity and target gene expression. A: Western blot analysis of ADRP overexpression showing endogenous rat ADRP and overexpressed murine FLAG-tagged ADRP, which ran approximately 2 kDa higher during electrophoresis. B: Incorporation of [1-14C]oleate into cellular lipids. C–E: Chase experiments evaluating turnover of TAG, phospholipids, and cholesterol esters. Statistics for the chase period were analyzed as a percentage of the pulse. F: PPARα reporter gene activity following exposure to fatty acids. G: Expression of PPARα target genes. All data are reported as means ± SE from three to four experiments. *P < 0.05 and **P < 0.01 when compared with Ad-GFP controls.