Fig. 4.

Overexpressing ATGL enhances the loss of fatty acids from TAG and increases PPARα activity and target gene expression. A: Western blot analysis of ATGL overexpression. B: Incorporation of [1-¹⁴C]oleate into cellular lipids. C: Chase experiments evaluating turnover of TAG. Statistics for the chase period were analyzed as a percentage of the pulse. D: PPARα reporter gene activity following exposure to fatty acids. E: Expression of PPARα target genes. All data are reported as means ± SE, n = 3. *P < 0.05 and **P < 0.01 when compared with Ad-GFP controls.