Table 1. Immune-related ORESTES generated from Biomphalaria glabrata.
Putative immune-factors as identified by BLAST searches (e < 10−5). The number of ORESTES recovered from each of the transcriptomes are indicated. The unknown category includes sequences that did not yield significant BLAST scores but that have similarity to transcripts reported as immune-relevant genes in other studies.
| #ESTs | |||||
|---|---|---|---|---|---|
| Characteristic or function | Sequence Product ID | Control | G+ -exposed | G− -exposed | S.m.- exposed |
| Pattern recognition/ binding | Angiopoietin-like 1 Fibrinogen-like | 1 | 0 | 0 | 0 |
| Beta-1,3-glucan-binding protein | 1 | 3 | 0 | 1 | |
| Calcium binding protein† | 0 | 5 | 0 | 0 | |
| Cellulase | 0 | 0 | 2 | 3 | |
| Chitin binding Peritrophin-A | 0 | 1 | 0 | 0 | |
| Chitin-binding protein | 0 | 1 | 1 | 0 | |
| Chitotriosidase | 1 | 1 | 0 | 1 | |
| C-type lectin‡ | 4 | 0 | 0 | 2 | |
| DEC-1† | 2 | 1 | 1 | 2 | |
| DEC-3† | 0 | 0 | 0 | 1 | |
| Fibrillin Biomphalaria glabrata chitin-binding† | 4 | 0 | 0 | 1 | |
| FREP 20 | 0 | 2 | 1 | 0 | |
| FREP 21 | 1 | 0 | 0 | 0 | |
| FREP 23 | 0 | 1 | 0 | 0 | |
| FREP 24 | 1 | 0 | 0 | 0 | |
| FREP 25 | 0 | 0 | 0 | 1 | |
| FREP 26 | 0 | 0 | 1 | 0 | |
| FREP 3 † | 0 | 1 | 0 | 0 | |
| FREP 5 | 0 | 0 | 1 | 0 | |
| FREP 7 | 0 | 0 | 0 | 1 | |
| Galectin | 2 | 1 | 0 | 1 | |
| Peptidoglycan-recognition protein (PGRP) | 0 | 1 | 0 | 0 | |
| Sialic acid binding lectin | 1 | 1 | 0 | 0 | |
| Thrombospondin-family Echinonectin | 0 | 0 | 0 | 1 | |
| Thrombospondin-family hemolectin | 1 | 0 | 0 | 1 | |
| Cell Adhesion | Cadherin Desmocollin | 0 | 1 | 0 | 0 |
| Cadherin Fat tumor suppressor protein‡ | 0 | 0 | 0 | 5 | |
| Dermatopontin 1 | 0 | 0 | 1 | 0 | |
| Dermatopontin A† | 1 | 1 | 3 | 0 | |
| Dermatopontin B | 1 | 0 | 0 | 0 | |
| Dermatopontin C | 0 | 1 | 0 | 0 | |
| Ependymin-related protein† | 1 | 3 | 3 | 0 | |
| Fatty acid binding protein | 0 | 1 | 1 | 0 | |
| Hyaluronan binding protein | 0 | 1 | 0 | 0 | |
| Integrin | 0 | 0 | 0 | 1 | |
| Matrilin† | 1 | 2 | 1 | 0 | |
| Signal transduction | DCC-interacting protein | 0 | 0 | 1 | 0 |
| Diguanylate cyclase/phosphodiesterase | 0 | 0 | 0 | 1 | |
| F-box-like/WD repeat protein repeat area of protein | 0 | 0 | 0 | 1 | |
| Frizzled | 1 | 0 | 0 | 0 | |
| Guanine nucleotide-binding protein | 1 | 0 | 1 | 1 | |
| Hepatocyte growth factor | 0 | 0 | 0 | 1 | |
| Hypocretin receptor 2 | 0 | 0 | 0 | 1 | |
| IkappaB kinase complex-associated protein | 0 | 1 | 0 | 0 | |
| MAP kinase-interacting serine/threonine kinase 2 | 2 | 0 | 0 | 0 | |
| Nucleoporin (nuclear pore complex protein, I-kappaB kinase regulation) | 0 | 0 | 1 | 1 | |
| Protein phosphatase | 0 | 1 | 0 | 0 | |
| Ras-like protein GTP-binding nuclear protein Ran (GTPase Ran) | 0 | 2 | 0 | 4 | |
| Ras-like protein rac 1 | 1 | 0 | 0 | 0 | |
| Ras-like protein Ran-binding protein 7 (Importin 7) | 0 | 0 | 1 | 0 | |
| Ras-like protein Ras-related protein Rab 1 | 1 | 1 | 2 | 1 | |
| Ras-like protein Ras-related protein Rab 6 | 1 | 0 | 0 | 0 | |
| Ribosomal protein S6 kinase | 0 | 1 | 0 | 0 | |
| Scr-like A-2 | 1 | 0 | 0 | 0 | |
| Signal transducer and activator of transcription | 1 | 0 | 0 | 0 | |
| Thrombospondin-family Bone morphogenetic protein | 0 | 0 | 0 | 1 | |
| Tumor necrosis factor, alpha-induced protein 3 | 1 | 0 | 0 | 0 | |
| Ubiquitin | 1 | 0 | 0 | 0 | |
| Ubiquitin Activating enzyme (E1) | 0 | 1 | 0 | 0 | |
| Ubiquitin Bg poly-ubiquitin gene | 3 | 2 | 1 | 2 | |
| Ubiquitin Conjugating enzyme | 0 | 0 | 1 | 1 | |
| Ubiquitin ligase | 2 | 0 | 0 | 0 | |
| Ubiquitin Specific protease 9 | 1 | 0 | 0 | 0 | |
| Ubiquitin Specific proteinase 34 | 0 | 0 | 0 | 1 | |
| Ubiquitin Ubiquitin conjugating enzyme | 0 | 1 | 0 | 0 | |
| vav 3 oncogene | 1 | 0 | 0 | 0 | |
| Antioxidant related | Catalase | 1 | 0 | 0 | 0 |
| Chorion oxidase/peroxidase | 3 | 1 | 0 | 3 | |
| Peroxinectin | 0 | 0 | 0 | 2 | |
| Peroxiredoxin | 0 | 1 | 2 | 0 | |
| Superoxide dismutase [Cu-Zn] | 4 | 0 | 0 | 1 | |
| Thioredoxin | 1 | 1 | 2 | 0 | |
| Apoptosis related | 24-dehydrocholesterol reductase | 1 | 0 | 0 | 0 |
| Amyloid beta binding protein Ubiquitin-Like | 0 | 0 | 0 | 1 | |
| Calreticulin | 1 | 0 | 0 | 0 | |
| Cullin 1 | 1 | 0 | 0 | 0 | |
| Cyclin | 1 | 0 | 0 | 0 | |
| Death-associated protein kinase | 0 | 0 | 0 | 1 | |
| Discoidin-Coagulation factor | 0 | 0 | 1 | 0 | |
| Endonuclease G | 4 | 0 | 1 | 1 | |
| Glyceraldehyde-3-phosphate dehydrogenase | 0 | 4 | 3 | 0 | |
| Inhibitor of apoptosis protein (IAP) | 2 | 0 | 0 | 1 | |
| Metabotropic X receptor | 0 | 2 | 0 | 0 | |
| Neurogenic locus notch homolog protein | 1 | 0 | 0 | 0 | |
| Neutral ceramidase | 0 | 0 | 1 | 0 | |
| p53 inducible protein isoform 1 | 1 | 0 | 0 | 1 | |
| Peflin (Penta-EF hand domain-containing protein 1) | 0 | 0 | 0 | 1 | |
| Phosphoinositide-3-kinase | 0 | 0 | 0 | 1 | |
| Protein tyrosine phosphatase | 1 | 0 | 0 | 0 | |
| Sphingomyelin phosphodiesterase | 0 | 0 | 2 | 1 | |
| Syntaxin-7 | 0 | 2 | 0 | 0 | |
| Thrombospondin-family Bg thrombospondin domain | 3 | 0 | 1 | 1 | |
| Thrombospondin-family Bg VWD domain-containing protein† | 4 | 1 | 3 | 1 | |
| Thrombospondin-family Hemocytin | 1 | 1 | 2 | 0 | |
| Thrombospondin-family Mucin 19 | 3 | 0 | 0 | 0 | |
| Thrombospondin-family Mucin 4 | 1 | 0 | 0 | 0 | |
| Thrombospondin-family Thrombospondin† | 0 | 0 | 2 | 0 | |
| Thrombospondin-family Zonadhesin† | 1 | 0 | 0 | 0 | |
| Translationally controlled tumor protein | 1 | 0 | 0 | 0 | |
| WD repeat protein | 2 | 2 | 0 | 1 | |
| Stress response | AMPK-related protein kinase | 0 | 0 | 0 | 1 |
| Heat shock protein 60 | 1 | 0 | 0 | 0 | |
| Heat shock protein 1 | 1 | 5 | 5 | 1 | |
| Heat shock protein 70 | 1 | 2 | 4 | 8 | |
| Heat shock protein 90 | 1 | 0 | 0 | 0 | |
| Heat shock protein beta-1 * | 1 | 0 | 0 | 0 | |
| Proteinase inhibitor I25 Cystatin | 0 | 0 | 0 | 1 | |
| Serine/threonine protein kinase | 1 | 2 | 3 | 2 | |
| Stress-induced-phosphoprotein 1 | 0 | 1 | 0 | 0 | |
| TFIIH basal transcription factor | 1 | 0 | 0 | 0 | |
| Universal stress protein | 0 | 1 | 1 | 0 | |
| Metal binding | Amiloride-sensitive amine oxidase | 1 | 0 | 1 | 2 |
| Copper-transporting ATPase | 0 | 0 | 0 | 1 | |
| Cysteine-rich motor neuron | 0 | 2 | 1 | 0 | |
| Cytochrome oxidase subunit II (COII, mitochondrial) | 1 | 8 | 7 | 1 | |
| Cytochrome P450* | 1 | 0 | 0 | 2 | |
| Dihydropyrimidinase | 1 | 0 | 1 | 0 | |
| Ferritin Soma 1 § | 5 | 2 | 0 | 9 | |
| Ferritin Soma 2 | 2 | 11 | 5 | 2 | |
| Ferritin Yolk 1 | 0 | 0 | 1 | 0 | |
| Ferritin Yolk 2 | 1 | 0 | 0 | 0 | |
| Hemoglobin type 1 | 23 | 30 | 45 | 42 | |
| Hemoglobin type 2† | 1 | 2 | 5 | 1 | |
| Iron responsive element binding protein | 1 | 0 | 0 | 0 | |
| Monooxygenase DBH-like 1 | 5 | 0 | 2 | 0 | |
| Myoglobin | 3 | 13 | 13 | 4 | |
| NADH dehydrogenase | 2 | 0 | 0 | 1 | |
| Phenylalanine hydroxylase | 0 | 0 | 1 | 0 | |
| Serine/threonine protein phosphatase PP1-beta | 1 | 0 | 0 | 1 | |
| Serpins | Serine Proteinase Inhibitors (serpins)(antithrombin) | 2 | 2 | 2 | 0 |
| Serine Proteinase Inhibitors (serpins)(antithrombin) Antithrombin-III | 1 | 3 | 1 | 0 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) BG serine protease alpha | 4 | 0 | 0 | 0 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) clade B (ovalbumin) | 1 | 0 | 2 | 1 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) fibrinolytic protease | 1 | 0 | 0 | 4 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) kazal-type proteinase inhibitor | 1 | 0 | 0 | 0 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) Serpin | 0 | 1 | 0 | 0 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) Serpin B | 0 | 1 | 0 | 1 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) Serpin I4 ‡ | 3 | 2 | 3 | 1 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) SerpinF | 0 | 0 | 0 | 1 | |
| Serine Proteinase Inhibitors (serpins)(antithrombin) Thrombospondin 2 | 1 | 0 | 0 | 0 | |
| Proteases | Cathepsin B | 1 | 4 | 0 | 0 |
| Cathepsin C | 0 | 0 | 2 | 0 | |
| Cathepsin D | 2 | 1 | 2 | 2 | |
| Cathepsin F | 0 | 0 | 0 | 1 | |
| Cathepsin L† | 2 | 3 | 4 | 1 | |
| Cathepsin Z | 0 | 2 | 0 | 0 | |
| Inflammatory response | ADAM | 1 | 0 | 1 | 1 |
| ADAM 17-like protease | 2 | 0 | 0 | 0 | |
| Secreted protein acidic and rich in cysteine (SPARC) | 0 | 3 | 0 | 0 | |
| Tryptase-2 | 0 | 0 | 1 | 0 | |
| Vacuolar protein sorting protein | 1 | 0 | 0 | 0 | |
| Other putative immune factors | Alpha-2-macroglobulin | 1 | 0 | 3 | 0 |
| Aplysianin-A‡ | 5 | 1 | 2 | 6 | |
| Bactericidal permeability-increasing protein (BPI)/Lipopolysaccharide-binding protein (LBP) | 0 | 0 | 0 | 1 | |
| Beta-1,4-endoglucanase 1†# | 2 | 0 | 0 | 2 | |
| Beta-catenin | 0 | 0 | 1 | 0 | |
| Contactin-associated protein-like 2 | 1 | 1 | 0 | 3 | |
| Complement: Utp21 specific WD40 | 0 | 0 | 1 | 0 | |
| Complement control protein containing SUSHI repeat | 1 | 0 | 0 | 0 | |
| Defense protein | 0 | 1 | 0 | 0 | |
| Developmentally regulated albumen gland gene protein | 21 | 2 | 6 | 2 | |
| Endo-1,4-mannanase† | 1 | 0 | 0 | 1 | |
| Fibulin | 1 | 1 | 0 | 1 | |
| G-type lysozyme | 1 | 0 | 0 | 0 | |
| IMMUNE-RESPONSIVE PROTEIN 1 | 1 | 0 | 0 | 0 | |
| Lipase | 3 | 0 | 0 | 0 | |
| Low density lipoprotein receptor-related protein | 4 | 0 | 1 | 2 | |
| Piwi-like | 0 | 0 | 0 | 1 | |
| S-adenosylhomocysteine hydrolase# | 0 | 1 | 0 | 0 | |
| Zinc Carboxypeptidase A# | 1 | 0 | 0 | 1 | |
| Cystatin, type 2 | 0 | 0 | 0 | 1 | |
| Unknowns | |||||
| Ucluster0156* | 0 | 0 | 4 | 0 | |
| UCluster0465* | 0 | 1 | 1 | 0 | |
| UCluster0600* | 0 | 0 | 2 | 0 | |
| UCluster0061† | 0 | 2 | 1 | 1 | |
| UCluster0007§ | 19 | 12 | 5 | 13 | |
| UCluster0525* | 0 | 0 | 2 | 0 | |
| P021C02§ | 0 | 0 | 0 | 1 | |
| P069B06* | 0 | 1 | 0 | 0 | |
†
Significantly upregulated in Biomphalaria glabrata susceptible to Echinostoma caproni(Bouchut et al. (2006a, b))
‡
Significantly upregulated in B. glabrata resistant to E. caproni (Bouchut et al. (2006a, b))
*
Recovered from a supression substractive hybridization library enriched for Schistosoma mansoni-exposed BS90 B. glabrata (Nowak et al. (2004))
#
Recovered from a supression substractive hybridization library enriched for E. paraensei-exposed M-line B. glabrata (Jung et al. (2005))
§
Recovered from a supression substractive hybridization library enriched for B. glabrata resistant to S. mansoni (Lockyer et al. (2007))