Figure 2.

Crystal Structure of Fibrillin-1 cbEGF9-hyb2-cbEGF10

Crystals were grown in the presence of 20 mM CaCl₂ to saturate the Ca²⁺ binding sites of both cbEGF domains. The cbEGF domains are shown in green and the hyb2 domain in magenta. Ca²⁺ bound to the cbEGF domains is represented by red spheres and disulphide bonds are shown in yellow. (A) Views either down the molecule (N terminus view) or from above the molecule (top view) show the overall linear arrangement of the domains. Ca²⁺ coordination in cbEGF9 (B) and cbEGF10 (C) was through conserved backbone and side-chain interactions as seen in previously determined structures (Abbott et al., 2007; Cordle et al., 2008; Lee et al., 2004; Rao et al., 1995).