Fig. 2.
Neutrophil superoxide anion production. One day after injury, blood was drawn via cardiac puncture and neutrophils were isolated. Neutrophil (5 × 106 cells/ml) O2− production was measured after stimulation with phorbol myristate acetate (PMA) (500 ng/ml) by using cytochrome c reduction assay. Plates were read at an outer diameter (OD) of 550 nm after every 1 min for 60 min. The peak O2− concentration was achieved ∼20–25 min after neutrophil stimulation with PMA. These peak values were recorded and pooled and are shown as means ± SE from at least 6 animals in each group. *P < 0.05 compared with sham and EtOH + Burn + anti-IL-18.