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Neutrophil intracellular elastase levels. One day after injury, blood was drawn via cardiac puncture and neutrophils were isolated. Neutrophil (2.5 × 106 cells/ml) elastase was measured after stimulation with PMA (500 ng/ml) at 37°C for 1 h. Cells were washed and lysed, and supernatant was collected for the estimation of intracellular elastase. Data are shown as means ± SE of fold increase from at least 6 animals in each group. *P < 0.05 compared with sham and EtOH + Burn + anti-IL-18.
