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. 2009 May 28;297(2):G406–G417. doi: 10.1152/ajpgi.00059.2009

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Fig. 5. — Long-term effects of HIS, 5-HT, and SOM on spinal neuronal characteristics. A: qPCR analysis revealed increased mRNA levels for SOM, both CGRP isoforms, and substance P after long-term incubation with HIS and 5-HT. Coincubation with HIS, 5-HT, and SOM still increased mRNA levels for all peptides investigated, but mRNA levels were reduced compared with the HIS- and 5-HT-incubated dishes. *Statistically significant difference compared with nonstimulated control dishes (P < 0.05); ‡statistically significant difference in HIS+5-HT+SOM stimulated dishes compared with HIS+5-HT stimulated dishes (P < 0.05). B: effect of long-term incubation with HIS, 5-HT, and SOM on the percentages of neurons expressing substance P, CGRP, or SOM. As for the qPCR experiments depicted in A, HIS- and 5-HT-incubation increased the percentages of spinal neurons expressing CGRP, substance P, or SOM. Coincubation with HIS, 5-HT, and SOM still increased the percentages of immunoreactive neurons compared with control dishes, but the percentages were lower than in HIS- and 5-HT incubated dishes. *Statistically significant difference compared with nonstimulated control dishes (P < 0.05); ‡statistically significant difference HIS+5-HT+SOM-stimulated dishes compared with HIS+5-HT-stimulated dishes (P < 0.05).