Table 1.
Primary, secondary, and tertiary antisera used for immunocytochemical stainings
| Antigen | Host | Dilution | Source | |||
|---|---|---|---|---|---|---|
| Primary Antisera | ||||||
| CGRP | Rabbit | 1/5,000 | Sigma, St. Louis, MO (C8198) | |||
| Glutamine synthetase | Rabbit | 1/20,000 | Abcam, Cambridge, UK (ab49873) | |||
| PECAM-1 | Goat | 1/500 | Santa Cruz Biotechnology, Santa Cruz, CA (sc-1506) | |||
| PGP | Guinea pig | 1/500 | GeneTex, San Antonio, TX (GTX10410) | |||
| SOM | Rat | 1/100 | Biogenesis, Poole, UK (8330-0009) | |||
| SSTR1 | Rabbit | 1/500 | Biotrend, Köln, Germany (SS-840) | |||
| SSTR2A | Rabbit | 1/2,000 | Biotrend (SS-800) | |||
| SSTR4 | Rabbit | 1/500 | Sigma (S0945) | |||
| Substance P | Rat | 1/100 | Biogenesis (8450-0505) | |||
| Secondary and Tertiary Antisera | ||||||
| Cy-3-conjugated donkey-anti-rabbit IgG | 1/1,000 | Jackson Immunoresearch, West Grove, PA | ||||
| Cy-3-conjugated donkey-anti-goat IgG | 1/500 | Jackson Immunoresearch | ||||
| Cy-3-conjugated donkey-anti-guinea pig IgG | 1/500 | Jackson Immunoresearch | ||||
| FITC-conjugated goat anti-rabbit IgG | 1/400 | Jackson Immunoresearch | ||||
| Biotinylated Fab fragments of goat-anti-rabbit IgG | 1/2,000 | Rockland, Gilbertsville, PA | ||||
| FITC-conjugated streptavidin | 1/100 | Rockland | ||||
| Extravidin horseradish peroxidase | 1/1,000 | Sigma | ||||
| Biotin-conjugated tyramide signal amplification kit | PerkinElmer Life Sciences, Boston, MA | |||||