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. 2009 Jun 3;297(2):C471–C480. doi: 10.1152/ajpcell.00019.2009

Fig. 5.

Fig. 5.

Tissue inhibitors of metalloproteinase (TIMP)-2, TIMP-3, and TIMP-4 inhibit angiogenesis and stabilize neovessels, whereas TIMP-1 stabilizes neovessels but promotes angiogenesis. A: angiogenesis in collagen gel cultures of rat aorta was inhibited by TIMP-2, TIMP-3, TIMP-4, and the synthetic MMP inhibitor GM6001 but not by TIMP-1, which had a proangiogenic effect. Change in angiogenic response at the peak of microvessel growth is expressed as percentage of control. When treatment was started following the angiogenic growth phase (days 5 and 6), vessels were effectively stabilized by the synthetic MMP inhibitor GM6001 (B), TIMP-2 (D), TIMP-3 (E), and TIMP-4 (F) and to a lesser degree by TIMP-1 (C). As a result, GM6001- or TIMP-treated neovessels survived longer than untreated control neovessels. Values are means ± SE; n = 4. *P < 0.05; **P < 0.01; ***P < 0.001.