Fig. 2.

Effects of 5-HT on reactive oxygen species (ROS) production in the absence or presence of ROS inhibitors. Compared with unstimulated cells (A), activation of smooth muscle cells with 5-HT (10 μM, 30 s) increased the ROS production (B) as evidenced by an increase in intensity of the DCF signal. Pretreatment with N-acetylcysteine (NAC) (C and D) or diphenyl iodonium (DPI) (E and F) inhibited 5-HT-induced ROS generation in cells. C and E, unstimulated cells; D and F, stimulated cells. Images are representative of 3 identical experiments.