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. 2009 May 20;297(2):C278–C289. doi: 10.1152/ajpcell.00093.2009

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Copyright © 2009, American Physiological Society

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Fig. 3. — NHE1 and NHE2 distribution between luminal and abluminal membranes of rat brain microvascular endothelial cells: quantitation of immunoelectron micrograph gold particles. Immunoelectron micrographs generated using NHE1 or NHE2 antibodies were evaluated for relative distribution of NHE isoforms in luminal (L) and abluminal (A) membranes of brain microvascular endothelial cells in perfusion-fixed rat brains. Values are means ± SE of 26 and 24 microvessels for NHE1 antibody dilutions of 1:1,000 and 1:2,000, respectively, and 56 and 81 microvessels for NHE2 antibody dilutions of 1:500 and 1:1,000, respectively.