Expression and distribution of matriptase and HAI-1 in the human prostate. Paraffin-embedded human prostate tissue sections were stained by immunohistochemistry using three mAbs: the M32 for total matriptase (A and B), the M69 for activated matriptase (E and F), and the M19 for HAI-1 (C and D). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. The photomicrographs in A, C, and E were taken from the ductal portions of the prostate and in B, D, and F from the acinial portions. Bar = 25 μm.