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Activated matriptase in HAI-1 complexes in human stomach. Normal human stomach tissue was homogenized using RIPA buffer. The lysate was incubated with the matriptase mAb 21-9-Sepharose beads to precipitate total matriptase. The eluted matriptase species were analyzed by immunoblotting for total matriptase using the mAb M32 (lane 1), activated matriptase using the mAb M69 (lane 2), and HAI-1 using the mAb M19 (lane 3).
