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COX-2 induction by Ti is absent in mIκB FLS. Wild-type FLS and stably transfected mIκB FLS were treated with Ti particles (5 × 106 particles/ml) for 0, 1, 2, or 4 h. The translocation of NF-κB (p65) was detected by immunofluorescence using anti-p65 antibody and a tetramethyl isothiocyanate (TRITC)-conjugated secondary antibody. DAPI was used as a nuclear stain.
