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PGE2 rescues receptor-activated NF-κB ligand (RANKL) expression in mIκB FLS. Subconfluent wild-type and mIκB FLS were treated with control media (C), titanium particles (5 × 106 particles/ml), or PGE2 (1 μM) for 24 h. Total protein extracts were harvested, and RANKL expression was examined by Western blot as described in experimental procedures. β-Actin was used as the loading control.
