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Effect of temperature and activase concentration on the activation state of Rubisco in vitro. Fully carbamylated Rubisco was incubated at the indicated temperatures in the absence (○) or presence of 0.025 (●), 0.05 (■), or 0.125 (▴) mg ml−1 activase under catalytic conditions. After 1.5 min, the fraction of catalytically competent sites was determined by measuring Rubisco activity. At all temperatures, Rubisco deactivation was linear with time for the first 2 min.
