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. 2009 May 26;297(2):E331–E339. doi: 10.1152/ajpendo.00133.2009

Fig. 4.

Fig. 4.

Erk1/2 inhibition blocks the combined stimulatory effect of estrogen and EGF on cell proliferation and PRL gene expression. A: GH3 cells were treated with E2 (0.01 nM), EGF (5 ng/ml), or their combination alone or in presence of UO126 (10 μM), and cell proliferation was determined by the MTT assay after 5 days. Data were calculated as percentages of vehicle control and presented as the means ± SE of 3 experiments. *Significant differences from control; **significant difference from E2 and EGF alone; ***significant difference from treatments without UO126 (P < 0.05). B: GH3 cells transiently cotransfected with PRL reporter gene and control reporter gene were treated with E2 (0.01 nM), EGF (5 ng/ml), or their combination, either alone or in presence of UO126 (10 μM), for 24 h, and normalized luciferase activity was determined as described in materials and methods. Data were calculated as fold change over control (arbitrary value of 1). Each value is the mean ± SE of 3 separate experiments, each performed in triplicate.