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Blockade of SHH pathway on wound healing in normal mice. The solution of SHH (5–20 μg/ml) or selective SHH receptor inhibitor cyclopamine (5 nmol/l) was directly placed on the wound surface for 30 min every other day from day 1 after wounding. The rate of wound closure was monitored every other day by tracing the wound area. Values were presented as means ± SE; n = 5. *P < 0.05 vs. control.
