Table 1.
Primer and probe sequences used for real-time PCR
| Variable | Primers and Probes |
|---|---|
| 9 | |
| Forward | AGGGCATGGATGAAGACAAACC |
| Reverse | TTTTCGGTGTTGACAGACTCAGT |
| FAM probe | TTGTGGGCATGCTCATGTT |
| 9 + 9* | |
| Forward | AGGGCATGGATGAAGACAAACC |
| Reverse | CCAAGCAAATTTCCCTTTCTTCTGAT |
| FAM probe | CTGGAGCGCCTCTGTTT |
| 21 | |
| Forward | ACATTGTTTTTACCACCATTTTCACCAT |
| Reverse | GCCCTTGTGCAGGAAAGC |
| FAM probe | CCGTAAGCAGTCATCTTT |
| 22 | |
| Forward | CAATGCAGACTATGTCTTCACTAGTATCT |
| Reverse | GCCCTTGTGCAGGAAAGC |
| FAM probe | CCGTAAGCAGTCATCTTAA |
| 32–33 | |
| Forward | GATTGTTGTGGGTAGCATTGTTGAT |
| Reverse | GATGGAGATGCGGGAGTTCT |
| FAM probe | CTCTGCACTGTGTACCTC |
| 31 + 33 | |
| Forward | CGTCATTGGGAGCATAATTGATGTC |
| Reverse | CACTCATAGAGGGAGAGCATTGG |
| FAM probe | ACTAATCCAGCTGAACATAC |
| 32 + 33 | |
| Forward | GATTGTTGTGGGTAGCATTGTTGAT |
| Reverse | CACTCATAGAGGGAGAGCATTGG |
| FAM probe | TACACCCAGCTGAACATA |