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. 2009 Jun 19;297(2):H533–H539. doi: 10.1152/ajpheart.00237.2009

Fig. 5.

Fig. 5.

Expression of focal adhesion associated proteins vinculin and paxillin is lower in Abl KO mice. A: blots of extracts from mesenteric arteries and aortae from Abl KO and WT mice were detected with use of antibodies against vinculin, paxillin, or smooth muscle-specific α-actin. Two samples of each treatment are shown in the blots. Vinculin-to-actin (vinculin/actin) (B) and paxillin-to-actin (paxillin/actin) (C) ratios of each artery from KO mice are normalized to those of corresponding artery from WT mice. *P < 0.05 (n = 7 to 8), significantly lower proteins ratios from Abl−/− mice compared with Abl+/+ mice. D: the densitometric level of actin in vascular tissues from the KO mice is normalized to that in corresponding arteries from WT mice (n = 7 to 8). E: RT-quantitative PCR was used to assess mRNA levels of vinculin and paxillin of Abl−/− and Abl+/+ mice. GAPDH mRNA was also determined to serve as a control. Ratios of vinculin/GAPDH and paxillin/GAPDH from Abl−/− mice are normalized to corresponding ratios of Abl+/+ mice. *P < 0.01 (n = 5), significantly lower ratios from Abl−/− mice compared with Abl+/+ mice. Values are means ± SE.