Fig. 2.

Increases in transmural pressure decrease myosin light chain 20 (MLC₂₀) phosphorylation in pressurized rat mesenteric lymphatics. A: representative blot for the MLC₂₀ reaction on lymphatic proteins isolated from lymphatics at different pressures. P-1, P-3, and P-7 indicate pressures of 1, 3, and 7 cmH₂O, respectively. B: representative blot for phosphorylated MLC₂₀ (pMLC₂₀). C: quantitative analyses of the pMLC₂₀-to-MLC₂₀ ratio. Signal intensities for MLC₂₀ and pMLC₂₀ from three different blots were used for the quantitative analyses. **P < 0.005 between the values at P-1 to P-3 and ***P < 0.001 between the values at P-1 to P-7.