Fig. 7.

Smad3 overexpression is associated with increased serine-10-phosphorylated p27 (S10-pp27). RSMCs were infected with AdSmad3 followed by stimulation with TGF-β₁ for 24 h. A: representative Western blot for p27 and S10-pp27. B: quantification of Western blot band intensity depicting ratio of S10-pp27 to total p27 after 24 h (*P < 0.05, n = 3 iterations of the experiment). C: injured rat carotid arteries infected with AdSmad3 or AdNull were harvested 14 days after injury, and protein was extracted for Western blot for p27, S10-pp27, and Smad3. A representative example from 3 separate Western blots shows that S10-pp27 levels are increased in arteries infected with AdSmad3 compared with control. Quantification of S10-pp27-to-total p27 (D) and total p27-to-β-actin (E) ratios in protein extracts from injured rat carotid arteries are shown (*P < 0.05, n = 3 rats).