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. Author manuscript; available in PMC: 2009 Aug 10.
Published in final edited form as: Physiol Genomics. 2005 Nov 29;24(3):276–289. doi: 10.1152/physiolgenomics.00109.2005

Fig. 3.

Fig. 3

LCM successfully excludes oligodendrocytes and astrocytes from captured neuronal populations. Quantitative real-time RT-PCR (qRT-PCR) was performed on spinal cord and LMN cDNA templates with primers and probes that specifically recognize classic myelin basic protein (MBP; oligodendrocytes) or glial fibrillary acidic protein (GFAP; astrocytes), and relative signal intensities were obtained. Spinal cord and LMN signals were normalized by the spinal cord signals. Normalized qRT-PCR signals reveal a 99.4 and 97% reduction in classic MBP and GFAP expression, respectively, in the LMN relative to the spinal cord template.