Fig. 2.

Expression of (P)RR in adult mouse forebrain by in situ hybridization with radiolabeled riboprobes (A and B) Low-magnification bright field images of mouse brain sections counterstained with toluidine blue after ISH with (P)RR antisense probe (A) or sense probe (B). A strong expression of (P)RR mRNA was detected in the cortex, the thalamus, and the pyramidal cell layer of CA3. A sense probe did not display any specific signal. C–N: higher magnification images showing the hybridization signals obtained with the antisense probe in the cerebral cortex (C–F; 1, 2, 4, 6b refer to the cortical layers and WM referes to the white matter), the hippocampal formation (F–K; PL refers to pyramidal cell layer in CA1, CA2, and CA3 fields; GL refers to granule cell layer in the dentate gyrus); the thalamus (K, L; DLG and VPL refer to dorsal lateral geniculate nucleus and ventral posteromedial thalamic nucleus, respectively); and the choroid plexus (M; LV indicates the lateral ventricle). Background levels obtained with the sense probe hybridized to a serial section are shown in for the dentate gyrus (J) and for the cortex (N), white matter, and CA1 field. Scale bar shown in N corresponds to 1,000 μm (A and B) and 60 μm (C–N).