Fig. 5.
Expression of COX-1 at the onset of LPS-induced hypothermia. COX-1 mRNA (A) and protein (B) were measured in spleen samples collected 50 min after intravenous administration of LPS (1,000 μg/kg) or saline at a subneutral Ta (22°C). A: mean relative expressions determined by quantitative real-time RT-PCR are shown on the left, whereas agarose-gel bands of amplicons obtained at the exponential phase of PCR amplification are shown on the right. B: mean contents (arbitrary units, AU) of COX-1 protein determined by densitometry of Western blot bands are shown on the left, whereas representative images of Western blot bands for spleen samples and platelet lysate (positive control) are shown on the right. Although β-actin expression was not examined in the Western blot analysis, it should be noted that an identical amount of total protein (35 μg) was loaded into each gel lane. Compared with saline, LPS did not increase the level of COX-1 mRNA or protein.