Fig. 4.
H2S regulates matrix metalloproteinase (MMP)-2 and -9 activities during hyperhomocysteinemia. In gel, gelatin zymography was performed to measure MMP-2 and -9 activities in the kidney cortex tissue-extracted protein. Both proforms and active forms of MMP-9 and active form of MMP-2 in WT 1-K, CBS(+/−) 2-K, and 1-K mice were found to be significantly higher than WT 2-K littermates. These MMP activities were attenuated by H2S supplementation (NaHS, 30 μmol/l) for the period of 8 wk postsurgery; data represent means ± SE, n = 5 for WT mice per group, and n = 6 for CBS(+/−) mice per group; *P < 0.01 vs. WT 2-K.