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cAMP activation enhances AQP2 transport activity in Xenopus laevis oocytes. Tracer urea uptake was measured into cRNA-injected oocytes in the absence of stimulators of cAMP or after incubation with a cocktail of 500 μM cyclopentyltheophylline (CPT)-cAMP, 500 μM IBMX, and 50 μM forskolin for 6 h. S256D and S256 are the phosphomimetic and phosphorylation-null mutants of AQP2, respectively.
