Fig. 5.
Inhibition of phosphodiesterase reduces the amplitude and frequency of SCcarb and enhances the inhibitory effect of SNAP. A: effect of 25 μM zaprinast on SCcarb. B: SNAP-mediated reduction of SCcarb in a vehicle-treated strip (0.1% DMSO; top) and in a strip pretreated with 25 μM zaprinast (bottom). C: concentration-response data for SNAP-mediated inhibition of SCcarb amplitude in control strips (without zaprinast) and in strips pretreated with 25 μM zaprinast (n = ≥6 for each concentration and treatment). D and E: summary data showing 100 μM SNAP-mediated inhibition of SCcarb amplitude (D) and frequency (E) in vehicle-treated control strips and strips pretreated with 25 μM zaprinast (n = 13). F: summary data comparing SCcarb amplitude with 100 μM SNAP, 100 μM 8-bromo-cGMP, and 100 μM SNAP after zaprinast application (n = ≥8 for each condition). *P < 0.05 between groups by 2-tailed t-test with layered Bonferroni correction.