Fig 6.

Interleukin (IL)-22 induces acanthosis, parakeratosis and downward epidermal projections in full-thickness skin equivalents. Haematoxylin and eosin staining of full-thickness human skin equivalents treated without (a) or with (b–d) the indicated cytokines. Blue arrow (d) indicates parakeratosis. Scale bars = 100 µm. (E) Reverse transcriptase–polymerase chain reaction quantification of gene expression normalized to HARP by full-thickness skin equivalents. Error bars indicate SD of triplicate samples. Data shown are representative of three experiments. Asterisks indicate statistical significance: *P < 0.05, **P < 0.01, ***P < 0.005.