Table 1.
Antibodies used for immunohistochemistry and immunofluorescence
Antigen | Manufacturer | Clonea | Isotype | Dilution | Amplification/detectionb |
---|---|---|---|---|---|
CD11c | BD Pharmingen, San Jose, CA, U.S.A. |
B-ly6 | IgG1 | 1:100 | Goat antimouse IgG1 (A568/A488) |
IL-17R | Amgen, Thousand Oaks, CA, U.S.A. |
M202 | IgG2a | 1:100 | Goat antimouse IgG2a (A488) |
IL-22R | R&D Systems, Minneapolis, MN, U.S.A. |
305405 | IgG1 | 1:100 | Goat antimouse IgG1 (A488) |
CXCR1 | eBioscience, San Diego, CA, U.S.A. |
8F1-1-4 | IgG2b | 1:100 | - |
CXCR2 | BD Pharmingen, San Jose, CA, U.S.A |
6C6 | IgG1 | 1:100 | - |
Vimentin | Neomarkers, Fremont, CA, U.S.A. |
V9 | IgG1 | 1:100 | Goat antimouse IgG1 (A568) |
All antibodies are murine monoclonals.
All amplification/detection antibodies are from Invitrogen/Molecular Probes (Carlsbad, CA, U.S.A.).