Table 2.
Antibodies used for flow cytometry
Antigen–fluorophore | Manufacturer | Clonea | Isotype | Dilution |
---|---|---|---|---|
IL-22 | R&D Systems, Minneapolis, MN, U.S.A. |
142928 | IgG1 | 1:33 |
CD3–Pacific Blue | eBioscience, San Diego, CA, U.S.A. |
OKT3 | IgG2a | 1:33 |
CD4–PE–Cy7 | eBioscience, San Diego, CA, U.S.A. |
RPA-T4 | IgG1 | 1:33 |
CD8–PerCP–Cy5.5 | BD Pharmingen, San Jose, CA, U.S.A |
SK1 | IgG1 | 1:20 |
IL-17–Alexa 488 | eBioscience, San Diego, CA, U.S.A. |
eBio64DEC17 | rat IgG2a | 1:20 |
IFN-γ–Alexa 700 | BD Pharmingen, San Jose, CA, U.S.A. |
B27 | IgG1 | 1:33 |
Live-Dead–aqua marina | Invitrogen, Carlbad, CA, U.S.A. |
NA | NA | 1:100 |
CD11c–PE | BD Pharmingen, San Jose, CA, U.S.A. |
S-HCL-3 | IgG2b | 1:33 |
CD45–PerCP | BD Pharmingen, San Jose, CA, U.S.A. |
2D1 | IgG1 | 1:20 |
IL-17R | Amgen, Thousand Oaks, CA, U.S.A. |
M202 | IgG2a | 1:33 |
IL-22R | R&D Systems, Minneapolis, MN, U.S.A. |
305405 | IgG1 | 1:33 |
All antibodies are murine monoclonals.