Figure 2.
Fluorescence anisotropy measurements with homologous nut-RNAs. 50 nM rrn boxA-spacer (filled square), λnutR boxA-spacer (filled circles), λnutL boxA-spacer (filled triangles) and λnutR boxB (open triangles) were titrated with NusA–SKK. The extrinsic fluorescence of the 3′ 6-FAM label of the RNAs was determined. The curves show the best fit to Equation (3) (see ‘Materials and Methods’ section). Kd values of 14 µM, 126 µM, 71 µM were determined, respectively (solid line; see Table 1). No Kd values could be fitted to λnutR boxB.