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. 2009 Jun 10;37(14):4736–4742. doi: 10.1093/nar/gkp452

Figure 3.

Figure 3.

Fluorescence anisotropy measurements with seperated RNAs regions. In each titration 50 nM of 6-FAM-labeled RNA was used. (A) 50 nM of 6-FAM-labeled λnutL boxA-spacer (squares), λnutL spacer (circles), λnutL boxA (triangles) were titrated with NusA-SKK. Kd values of 71 µM and 24 µM were determined for λnutL boxA-spacer, λnutL spacer, respectively (solid lines). No Kd value could be fitted to λnutL boxA (see Table 1). (B) 50 nM of 6-FAM-labeled λnutR boxA-spacer (circles), λnutR spacer (squares), λnutR boxA (triangles) were titrated with NusA-SKK. Kd values of 126 µM and 137 µM were determined for λnutR boxA-spacer, λnutR spacer, respectively (solid lines). No Kd value could be fitted to λnutR boxA (see Table 1). (C) 50 nM of 6-FAM-labeled rrn boxA alone (open triangle), rrn cac-boxA-spacer (open square), rrn spacer I (open circle), rrn spacer II (filled circle), rrn spacer III (filled triangle) were titrated with NusA-SKK. Kd values can be seen in Table 1. No Kd value could be fitted to rrn boxA (see Table 1).