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. 2009 Jun 5;37(14):4642–4656. doi: 10.1093/nar/gkp460

Figure 3.

Figure 3.

Deletion Tdt Mutants in Loop1. (A) The activity without CoCl2 but in the presence of 4 mM MgCl2 of (from left to right) wild-type Tdt, Δ384, Δ392, the double mutant Δ2383/392 and triple mutant Δ3383/391/392 is shown for each dNTP and a mixture of all dNTPs. (B) The effect of Co++ addition (1 mM) to the reaction buffer on the activity of selected mutants described in Figure 2 and Figure 3A.