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. 2009 Jun 5;37(14):4642–4656. doi: 10.1093/nar/gkp460

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Mimicking pol μ and pol λ with Tdt. (A) The activity without CoCl₂ but in the presence of 4 mM MgCl₂ of wild-type Tdt and Tdt Chimera containing the pol μ Loop1, as well as the Δ13 mutant (as in pol λ) is shown for each dNTP and a mixture of all dNTPs. (B) The effect of Co⁺⁺ addition (1 mM) to the reaction buffer is shown for the same mutants.