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. 2009 Jun 5;37(14):4642–4656. doi: 10.1093/nar/gkp460

Figure 5.

Figure 5.

SD1 region mutants (QKC). (A) The activity without CoCl2 but in the presence of 4 mM MgCl2 of wild-type Tdt, Q402E, Q402A, K403R, K403A and C404A mutants is shown for each dNTP and a mixture of all dNTPs. (B) The activity without CoCl2 but in the presence of 4 mM MgCl2 of Tdt wild-type, along with the triple mutant in SD1 switching QKC into ERS (as in pol μ), D399A, H400A, F401A and F405A mutants is shown for each dNTP and a mixture of all dNTPs. The effect of Co++ addition (1 mM) to the reaction buffer is shown in Supplementary Figure S2.