Figure 6.

Jdp2 isoforms localize to the nucleus. NIH3T3 cells were transiently transfected with vectors expressing N-terminally FLAG tagged full length Jdp2 (A) or either of three N-terminally Jdp2 isoforms, Jdp2-132 (B-C), Jdp2-119 (D) or Jdp2-107 (E). As controls are shown cells transfected with empty vector (F). The subcellular localization was revealed by immunocyotchemistry using an anti-FLAG primary antibody (left). Cells were co-stained with DAPI dye (right). The results are representative of three independent experiments.