Skip to main content
. 2009 Jun 22;37(14):4873–4886. doi: 10.1093/nar/gkp471

Figure 1.

Figure 1.

Example analysis of EST sequences. In this hypothetical example, the major isoform of a gene has six introns and seven ESTs have been observed in a library. Three of the ESTs sequences (EST3, EST4, EST5) contain alternative introns—introns that differ at the 3′ and/or 5′ end from corresponding intron in the major isoform. The fractional abundance of alternative transcripts is 42% (3 out of 7). The number of isoforms for this gene is 3 (major isoform, EST3 isoform and EST5 isoforms). EST4 is not counted as an additional isoform because it has the same pattern as EST3. There are a total of 13 detected splicing reactions (count of all introns from all ESTs) and 3 of these splicing reactions are classified as alternative. The implied error rate for this gene is 0.23 (3 out of 13 splicing reactions).